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mouse anti ddk flag monoclonal antibody  (OriGene)


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    Structured Review

    OriGene mouse anti ddk flag monoclonal antibody
    Mouse Anti Ddk Flag Monoclonal Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ddk+flag/Clone+OTI4C5%2C+Anti-DDK+(FLAG)+monoclonal+antibody/pmc13116172-168-20-26
    Average 96 stars, based on 363 article reviews
    mouse anti ddk flag monoclonal antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Bortezomib Is Effective in the Treatment of T Lymphoblastic Leukaemia by Inducing DNA Damage, WEE1 Downregulation, and Mitotic Catastrophe
    Article Snippet: .. The antibodies for the immunoblots, including GAPDH (14C10), P21 waf1/cip1 (12D1), P53, P27 kip1 (D69C12), CDK1 (POH1), CYCLIN B1 (D5C10), and WEE1 (D10D2), were purchased from Cell Signaling (Beverly, MA, USA), while Anti-DDK(FLAG) (TA50011-100) was purchased from OriGene Technologies, Inc. (Rockville, MD 20850, USA). ..

    Article Title: Interplay between ALK2 R206H mutant receptor and autophagy signaling regulates receptor stability and its chondrogenic functions.
    Article Snippet: .. For immunoblotting, 20–30 μg of protein extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), blotted on nitrocellulose membrane, and stained with the specific primary antibodies: anti-Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E,Cell Signaling Technology), anti-p62 (SQSTM1) (PM045; MBL International), anti-GAPDH (D16H11) (Cell Signaling Technology), anti-LC3B (Sigma–Aldrich, L7543), anti-NanoLuc (Promega Corporation, N700A), anti-DDK (FLAG) (Origene, TA50011-100), anti ALK2/ACVR1 (SinoBiological. .. 10227-T24), anti-SMAD1 (Cell Signaling Technology, 9743S), anti-phosphoSmad 1/5 (Ser463/465) (Cell Signaling Technology, D5B10), anti-phoshoSmad2 (Ser465/467)/Smad3 (Ser423/425) (Cell Signaling Technology, D27F4), anti-ATG5 (Proteinech, 10181-2-AP), anti-Rubicon (Proteinech 21444-1-AP),anti-tubulin (Sigma–Aldrich, 1:3000) and anti-Hsp90 (F8, Santa Cruz Biotechnology, 1/2000), anti-Vinculin (Santa-Cruz, SC-73614) Secondary antibodies were anti-mouse (Bio-Rad Laboratories, 1706515) or anti-rabbit (Bio-Rad Laboratories, 1706516) IgG-horseradish peroxidaseconjugated antibodies (Cell Signaling), incubated at a dilution of 1:4000.

    Article Title: Bortezomib Is Effective in the Treatment of T Lymphoblastic Leukaemia by Inducing DNA Damage, WEE1 Downregulation, and Mitotic Catastrophe.
    Article Snippet: .. The antibodies for the immunoblots, including GAPDH (14C10), P21waf1/cip1 (12D1), P53, P27kip1 (D69C12), CDK1 (POH1), CYCLIN B1 (D5C10), and WEE1 (D10D2), were purchased from Cell Signaling (Beverly, MA, USA), while Anti-DDK(FLAG) (TA50011-100) was purchased from OriGene Technologies, Inc. (Rockville, MD 20850, USA). ..

    Nucleic Acid Electrophoresis:

    Article Title: Interplay between ALK2 R206H mutant receptor and autophagy signaling regulates receptor stability and its chondrogenic functions.
    Article Snippet: .. For immunoblotting, 20–30 μg of protein extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), blotted on nitrocellulose membrane, and stained with the specific primary antibodies: anti-Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E,Cell Signaling Technology), anti-p62 (SQSTM1) (PM045; MBL International), anti-GAPDH (D16H11) (Cell Signaling Technology), anti-LC3B (Sigma–Aldrich, L7543), anti-NanoLuc (Promega Corporation, N700A), anti-DDK (FLAG) (Origene, TA50011-100), anti ALK2/ACVR1 (SinoBiological. .. 10227-T24), anti-SMAD1 (Cell Signaling Technology, 9743S), anti-phosphoSmad 1/5 (Ser463/465) (Cell Signaling Technology, D5B10), anti-phoshoSmad2 (Ser465/467)/Smad3 (Ser423/425) (Cell Signaling Technology, D27F4), anti-ATG5 (Proteinech, 10181-2-AP), anti-Rubicon (Proteinech 21444-1-AP),anti-tubulin (Sigma–Aldrich, 1:3000) and anti-Hsp90 (F8, Santa Cruz Biotechnology, 1/2000), anti-Vinculin (Santa-Cruz, SC-73614) Secondary antibodies were anti-mouse (Bio-Rad Laboratories, 1706515) or anti-rabbit (Bio-Rad Laboratories, 1706516) IgG-horseradish peroxidaseconjugated antibodies (Cell Signaling), incubated at a dilution of 1:4000.

    Membrane:

    Article Title: Interplay between ALK2 R206H mutant receptor and autophagy signaling regulates receptor stability and its chondrogenic functions.
    Article Snippet: .. For immunoblotting, 20–30 μg of protein extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), blotted on nitrocellulose membrane, and stained with the specific primary antibodies: anti-Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E,Cell Signaling Technology), anti-p62 (SQSTM1) (PM045; MBL International), anti-GAPDH (D16H11) (Cell Signaling Technology), anti-LC3B (Sigma–Aldrich, L7543), anti-NanoLuc (Promega Corporation, N700A), anti-DDK (FLAG) (Origene, TA50011-100), anti ALK2/ACVR1 (SinoBiological. .. 10227-T24), anti-SMAD1 (Cell Signaling Technology, 9743S), anti-phosphoSmad 1/5 (Ser463/465) (Cell Signaling Technology, D5B10), anti-phoshoSmad2 (Ser465/467)/Smad3 (Ser423/425) (Cell Signaling Technology, D27F4), anti-ATG5 (Proteinech, 10181-2-AP), anti-Rubicon (Proteinech 21444-1-AP),anti-tubulin (Sigma–Aldrich, 1:3000) and anti-Hsp90 (F8, Santa Cruz Biotechnology, 1/2000), anti-Vinculin (Santa-Cruz, SC-73614) Secondary antibodies were anti-mouse (Bio-Rad Laboratories, 1706515) or anti-rabbit (Bio-Rad Laboratories, 1706516) IgG-horseradish peroxidaseconjugated antibodies (Cell Signaling), incubated at a dilution of 1:4000.

    Staining:

    Article Title: Interplay between ALK2 R206H mutant receptor and autophagy signaling regulates receptor stability and its chondrogenic functions.
    Article Snippet: .. For immunoblotting, 20–30 μg of protein extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), blotted on nitrocellulose membrane, and stained with the specific primary antibodies: anti-Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E,Cell Signaling Technology), anti-p62 (SQSTM1) (PM045; MBL International), anti-GAPDH (D16H11) (Cell Signaling Technology), anti-LC3B (Sigma–Aldrich, L7543), anti-NanoLuc (Promega Corporation, N700A), anti-DDK (FLAG) (Origene, TA50011-100), anti ALK2/ACVR1 (SinoBiological. .. 10227-T24), anti-SMAD1 (Cell Signaling Technology, 9743S), anti-phosphoSmad 1/5 (Ser463/465) (Cell Signaling Technology, D5B10), anti-phoshoSmad2 (Ser465/467)/Smad3 (Ser423/425) (Cell Signaling Technology, D27F4), anti-ATG5 (Proteinech, 10181-2-AP), anti-Rubicon (Proteinech 21444-1-AP),anti-tubulin (Sigma–Aldrich, 1:3000) and anti-Hsp90 (F8, Santa Cruz Biotechnology, 1/2000), anti-Vinculin (Santa-Cruz, SC-73614) Secondary antibodies were anti-mouse (Bio-Rad Laboratories, 1706515) or anti-rabbit (Bio-Rad Laboratories, 1706516) IgG-horseradish peroxidaseconjugated antibodies (Cell Signaling), incubated at a dilution of 1:4000.

    other:

    Article Title: Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis
    Article Snippet: Anti-DDK (Flag) (TA50011-1), and anti-Myc (TA150121) were obtained from Origene (Rockville, MD, USA).

    Over Expression:

    Article Title: Aberrant N-glycosylation in pathogenic variants of adenosine deaminase 2 underlying human ADA2 deficiency
    Article Snippet: .. After overexpression, tagged ADA2 was pulled down using 1 μg anti-DDK (FLAG) clone OTI4C5 (#TA50011, OriGene Technologies). .. An isotype control sample was incubated with 1 μg mouse IgG1 (#02-6100; Invitrogen) in parallel.



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